🧪🌬️ SOP for Operation and Maintenance of Fume Hood

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🧪🌬️ SOP for Operation and Maintenance of Fume Hood

1. Purpose

To define the standardized procedure for the safe operation, monitoring, and maintenance of laboratory fume hoods to ensure protection of personnel, containment of hazardous fumes, and compliance with GMP and regulatory requirements.

2. Scope

  • Quality Control (QC) laboratories
  • Research and development laboratories
  • Any laboratory area using chemical fume hoods

3. Definitions

  • Fume Hood: A ventilated enclosure designed to capture, contain, and exhaust hazardous chemical vapors, fumes, and dust.
  • Sash: Movable glass panel that acts as a barrier between user and hood interior.
  • Face Velocity: Average air velocity entering the hood through the sash opening.
  • Exhaust System: Ducting and fan system that removes contaminated air from the hood.

4. Precautions

  • Always work with the sash at the recommended operating height.
  • Do not place head or body inside the fume hood.
  • Do not use the fume hood for storage of chemicals.
  • Avoid blocking air flow by placing large equipment near the front grille.
  • Wear appropriate PPE (lab coat, gloves, safety goggles).
  • Do not operate the hood if airflow alarm indicates malfunction.
  • Only trained personnel shall use the fume hood.

5. Responsibilities

Role Responsibility
QC Analyst / User Safe operation and daily checks
QC Supervisor Ensure compliance and review usage practices
Quality Assurance Audit oversight and deviation handling
Engineering / Maintenance Performance testing, maintenance, and certification

6. Equipment & Materials

  • Laboratory Fume Hood
  • Airflow Indicator / Alarm System
  • Anemometer (for airflow testing)
  • Approved Cleaning Materials
  • Authorized Inspection & Maintenance Records

7. Operation Procedure

  • Check that the fume hood airflow indicator shows normal operation.
  • Ensure sash glass is intact and clean.
  • Adjust sash to the recommended operating height (marked level).
  • Switch ON the hood and allow airflow stabilization.
  • Perform all chemical handling at least 15 cm inside the hood.
  • Keep containers closed when not in use.
  • Minimize rapid movements to avoid airflow disturbance.
  • After work completion, close chemical containers and lower the sash.
  • Switch OFF the hood if not required, as per site policy.

8. Maintenance & Performance Verification

  • Daily: Visual inspection of sash, airflow indicator, and cleanliness.
  • Monthly: Check alarms and airflow consistency.
  • Periodic (6–12 months): Face velocity testing using an anemometer.
  • Acceptable face velocity: 0.45–0.60 m/s (or as per site SOP).
  • Document all inspections and maintenance activities.

9. Deviations

  • If airflow is inadequate, stop work immediately.
  • Label hood as OUT OF SERVICE.
  • Inform supervisor and engineering.
  • Resume use only after corrective action and re-certification.

10. References

  • WHO Technical Report Series – GMP
  • ICH Q7, Q9, Q10
  • EU GMP Guidelines – EudraLex Volume 4
  • ISO 14644 (Cleanroom & Controlled Environments)
  • NIOSH / OSHA Laboratory Safety Guidelines
  • Manufacturer’s Instruction Manual – Fume Hood

© 2026 sopnest.blogspot.com. All rights reserved. This content is independently written and intended for educational purposes. References to regulatory guidelines are for compliance guidance only.

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